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KMID : 0352720110350040504
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Journal of Ginseng Research
2011 Volume.35 No. 4 p.504 ~ p.513
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Rapid Identification of Ginseng Cultivars (Panax ginseng Meyer) Using Novel SNP-Based Probes
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Jo Ick-Hyun
Bang Kyong-Hwan
Kim Young-Chang
Lee Jei-Wan
Seo A-Yeon
Seong Bong-Jae
Kim Hyun-Ho
Kim Dong-Hwi
Cha Seon-Woo
Cho Yong-Gu
Kim Hong-Sig
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Abstract
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In order to develop a novel system for the discrimination of five ginseng cultivars (Panax ginseng Meyer), single nucleotide polymorphism (SNP) genotyping assays with real-time polymerase chain reaction were conducted. Nucleotide substitution in gDNA library clones of P. ginseng cv. Yunpoong was targeted for the SNP genotyping assay. From these SNP sites, a set of modified SNP specific fluorescence probes (PGP74, PGP110, and PGP130) and novel primer sets have been developed to distinguish among five ginseng cultivars. The combination of the SNP type of the five cultivars, Chungpoong, Yunpoong, Gopoong, Kumpoong, and Sunpoong, was identified as 'ATA', 'GCC', 'GTA', 'GCA', and 'ACC', respectively. This study represents the first report of the identification of ginseng cultivars by fluorescence probes. An SNP genotyping assay using fluorescence probes could prove useful for the identification of ginseng cultivars and ginseng seed management systems and guarantee the purity of ginseng seed.
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KEYWORD
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Panax ginseng, Nucleotide substitution, Fluorescence probes, Single nucleotide polymorphism genotyping
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